MIARE 0.8.0 http://miare.sourceforge.net/HomePage#guidelines Taken direct from the web-available version 0.8.0 (PDF) on 2011-06-04 by Chris Taylor (chrisftaylor@gmail.com) A. Assay description A.1. Assay ID A.2. Assay name A.3. Assay Type primary/confirmatory/other A.4. Target organism Taxonomy ID A.5. Number of distinct genes targeted for knock-down A.6. Experiment publication (PubMed ID) PubMed ID A.7. Primary contact information B. Protocol B.2. Assay B.2.1. Assay protocol and design including number and description of replicates (biological/technical) B.2.2. Pre- and post-treatment protocol/type/compound B.2.3. Bio-material manipulations including growth conditions/cell culture conditions and if applicable cell separation technique B.2.4. Number of cells per well B.2.5. Compound(s) name if applicable shall B.2.5.1. Assay reagent name B.2.5.2. Assay reagent manufacturer B.2.6. Instrument B.2.6.1 Instrument name B.2.6.2. Instrument manufacturer B.2.6.3. Type of readout B.2.6.4. Instrument settings B.1. Experimental description B.1.1. Experiment title B.1.2. Biological question description including sample description and keywords B.3. Delivery B.3.1. Delivery type and protocol B.3.1.1. Percentage of cell confluence if applicable shall B.3.1.2. Complexing protocol B.3.1.3. Complexing Time B.3.2. Delivery reagent B.3.2.1. Delivery reagent type B.3.2.2. Delivery reagent manufacturer B.3.2.3. Delivery reagent name B.3.2.4. Delivery reagent final concentration B.3.3. Silencing reagent final concentration B.4. Silencing RNA reagent Substance B.4.1. Silencing RNA reagent ID B.4.1.1. Probe ID if applicable shall B.4.5. Silencing RNA reagent library description Provider/Version No. B.4.2. Target gene ID or accession number NCBI/EMBL/DDBJ B.4.3. Target gene name if available shall B.4.4. Silencing RNA reagent sequence(s) cross-reference to GenBank ID if available shall B.4.6. Silencing RNA reagent type if applicable shall B.4.7. Unique silencing RNA molecules per reagent pool if applicable shall B.4.8. Modifications to silencing RNA reagent if applicable shall B.4.10. Vector/Plasmid reference if applicable shall B.4.9. Taxonomy ID B.4.11. Comments B.5. Assay plate description B.5.1. Assay plate manufacturer B.5.2. Assay plate type B.6. Assay plate B.6.1. Media changes B.6.1.1. Media composition B.6.1.2. Time of media change C. Results C.1. Data analysis C.1.1. Bioactivity outcome threshold C.1.2. Bioactivity score assignment method C.1.3. Data normalisation method C.1.4. Artefacts C.1.5. Data filtering description C.1.6. Data transformation details C.1.7. Analysis program C.1.7.1. Analysis script description C.1.7.2. Analysis Software name/version C.1.8. Quantitative data C.1.8.1. Description of quantified data C.1.9. Qualitative data C.1.9.1. Description of qualitative data C.2. Result definitions C.2.1. Data column definitions for assay results C.2.1.1. Name C.2.1.2. Data type float, integer, string or NCBI Entrez database ID C.2.1.3. Unit C.2.1.4. Description C.2.1.5. Constraint min, max, range or set of values C.3. Data C.3.1. Quantitative data C.3.1.1. Unprocessed quantified data raw data if applicable shall C.3.1.2. Normalised quantified data C.3.1.3. Scored data C.3.1.3.1. Bioactivity outcome active, inactive, inconclusive C.3.2. Qualitative data C.3.2.1. Qualitative data